Composite

Part:BBa_K3838888:Design

Designed by: Siyang Yu   Group: iGEM21_SZU-China   (2021-08-30)


J-SOD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 394
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 394
    Illegal BglII site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 394
    Illegal NgoMIV site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some bacteria would express the SOD gene itself,which should be considered in the experiment.


Source

The SOD gene is from Lactobacillus casei strain Lc18.

References